Michael Jordan Essays (984 words) - Chicago Bulls,

Michael Jordan Michael Jordan was one of five children born to James and Delores Jordan. He was born February 17, 1963 in Brooklyn. His parents felt that the streets of Brooklyn were unsafe to raise a young family. So instead of trying to endure the streets of Brooklyn, the Jordan family moved to Wilmington, North Carolina. Michael's father, James, got a job in Wilmington as a mechanic and his mother Delores got a job as a teller at United Carolina Bank. Michael always had an eye for baseball. He played as an outfielder and as a pitcher. When he was twelve, he was the top player in his league. By the age of fifteen, he wasn't the star in baseball as he once was. He was still very good, but he had lost some of his focus. Later, in his high school career, he dropped baseball to pursue another interest. Soon Michael adopted the game of basketball. When Michael reached the ninth grade, he tried out for the basketball team. Coach Lynch, Michael's coach, cut Michael, which in turn may have made the best player alive today. Michael then took practicing basketball to another level. He played his brother Larry whenever he could. Michael never expected what would come in the near future. Michael Jordan went to the University of North Carolina as a basketball recruit. Even though Jordan at 6'5" was a man with potential, he still studied very hard in an attempt to get a good education, while competing in sports. Mike wasn't expected to be a star of the Tar Heels, since they had players such as James Worthy, Sam Perkins, and Al Wood. But, by the end of the 1981-82 season, Jordan, as a freshman, was an everyday starter. Carolina reached the Final Four with the help of Jordan, who had sixteen points and led the team with nine rebounds. In the final twenty seconds, Michael made a title winning shot from the left baseline. In Michael's sophomore and junior seasons, he was voted College Basketball's Player of the Year. Through Michael's three years at Carolina, he average 17.7 points per game, while maintaining a "B" average. After a great college career, the Chicago Bulls as the third pick of the 1984-85 season drafted Michael. Michael made a huge impact in the NBA (National Basketball Association). He made such an impact that he was selected to be an alternate on the Eastern Division all-star squad. With Michael's growing success in the NBA, more and more companies wanted to use the "Air Jordan" name. One of the first companies to use his name was Nike. Nike paid Michael $2.5 million, so they could begin a line of shoes known as "Air Jordan's ?During the 1987-88 season, Jordan raised his game to another level. The Bulls' status was also raised to another level with the additions of Scottie Pippen and Horace Grant. Michael was having another great season and this was evidenced when he started on the Eastern Division all-star squad.?(Website) He then finished the season, leading the Bulls in scoring for 81 games out of the 82 game season. For his efforts, Jordan was selected as the Most Valuable Player and as Defensive Player of the Year. Throughout the rest of the few years Chicago went on a rampage. They had received the best records in history, and with the help of Michael they went on to win three NBA championships. Michael Jordan announced after winning his third consecutive NBA championship that he was stepping down from the game he loved. He said that he was tired of the pressures. Some people believe that Jordan's gambling debts caused Jordan to quit. Others believe that the slaying of his father, which happened on August 15, 1993, caused Jordan to quit. There is one final theory that Michael wanted to pursue one of his childhood dreams, professional baseball. Michael Jordan announced that he would pursue his dream of playing baseball. Jordan joined the Chicago White Sox organization. The White Sox gave Jordan a tryout and then decided that he would be best playing with the Birmingham Barons. When Mike hit his first home run on July 30,he pointed to the sky honoring

Free Essays on Racial Discrimination During The 1920s

Racial and Ethnic Discrimination during the 1920's During the 1920’s, racial tensions in American society reached a boiling point. New non-protestant immigrants like Jews and Catholics had been arriving in huge masses from southeast Europe since the beginning of the century. Together, with Orientals, Mexicans, and the African-American population, these minorities suffered at the hands of those concerned with preserving the long established White Anglo-Saxon Protestant (W.A.S.P.) values that were an integral part of American life. Prejudice and racism reared its ugly head in many areas of society, with people showing a tolerance for racist views in the media, literature, and towards organizations like the Ku Klux Klan. Also, the language, living and working conditions, and Government legislations that ethnic minorities were subjected to is further evidence that the Twenties were an openly discriminatory decade. It was also during this period of grave hostility directed at ethnic groups that America’s ‘open door ’ attitude of â€Å"give me your tired, your poor†¦Ã¢â‚¬  officially became a part of its history. During the â€Å"Roaring Twenties†, anti-immigration organizations that had been founded in the 1900’s began to receive more support and became increasingly influential following the First World War. The Immigration Restriction League (I.R.L.) was one such group, which claimed to have scientific evidence that the new immigrants from southeast Europe were racially â€Å"inferior† and therefore posed a threat to the supremacy of the United States. They believed strongly in W.A.S.P. values and certainly did not wish to see them become polluted by other religions from minority groups. This Social-Darwinist belief was not just popular with the masses, but it’s appeal spread to people of considerable eminence. For example, the principals of important American universities like Harvard and Stanford were numbered among the I.... Free Essays on Racial Discrimination During The 1920's Free Essays on Racial Discrimination During The 1920's Racial and Ethnic Discrimination during the 1920's During the 1920’s, racial tensions in American society reached a boiling point. New non-protestant immigrants like Jews and Catholics had been arriving in huge masses from southeast Europe since the beginning of the century. Together, with Orientals, Mexicans, and the African-American population, these minorities suffered at the hands of those concerned with preserving the long established White Anglo-Saxon Protestant (W.A.S.P.) values that were an integral part of American life. Prejudice and racism reared its ugly head in many areas of society, with people showing a tolerance for racist views in the media, literature, and towards organizations like the Ku Klux Klan. Also, the language, living and working conditions, and Government legislations that ethnic minorities were subjected to is further evidence that the Twenties were an openly discriminatory decade. It was also during this period of grave hostility directed at ethnic groups that America’s ‘open door ’ attitude of â€Å"give me your tired, your poor†¦Ã¢â‚¬  officially became a part of its history. During the â€Å"Roaring Twenties†, anti-immigration organizations that had been founded in the 1900’s began to receive more support and became increasingly influential following the First World War. The Immigration Restriction League (I.R.L.) was one such group, which claimed to have scientific evidence that the new immigrants from southeast Europe were racially â€Å"inferior† and therefore posed a threat to the supremacy of the United States. They believed strongly in W.A.S.P. values and certainly did not wish to see them become polluted by other religions from minority groups. This Social-Darwinist belief was not just popular with the masses, but it’s appeal spread to people of considerable eminence. For example, the principals of important American universities like Harvard and Stanford were numbered among the I....

Free sample - Ethical Standards. translation missing

Ethical Standards. Ethical StandardsMoral and ethical aspects of human life are very important for the personal development and statement. These life values determine attitude towards people, God and family. They are not regulated by law or other governmental documents, and there is no determined punishment for their violation, but still breaking ethical rules means breaking own personality and acting against oneself. 1. Ethical standard in majority of cases is taken from the parents’ family, social life, religion and historical period. Also, some people think that social groups, mentality and corporate culture, make impact on ethical standards formation. In other words, it is possible to consider the social environment as a major factor, which influences the ethical and moral standards of the personality. 2. According to the respondents’ point of view, those standards of personal ethics, which were grounded by parents in the childhood, are completely impossible to change because personality makes all important life decisions and actions, according to these standards. In some cases, it is possible to make some impact for the personal ethics while changing environment and religious- person would have to adapt to the new life conditions and to the people, surrounding him or her with their life principles. 3. In most cases ethical standards consider God as the main judge of all our actions. The God is also represented for many people as the personal example on how people should live and act. Family values are the core principle of ethics and people think that it is impossible to be fair with him/herself without being fair in own family. Parents, kids and beloved are the sense of life. Belief in afterlife time – one more important issue in following all ethical norms and standards. Respondents consider the afterlife time as the remuneration for the fair and faithful life, for acting, according to the ethical norms. References: Auditing Practices Board (2010) Ethical Standards. [Online] (Updated 17 July 2010) World Health Organisation (2010) Ethical standards and procedures for research with human beings

Midwifery Code of Conduct Essay Example | Topics and Well Written Essays - 1500 words - 2

Midwifery Code of Conduct - Essay Example The healthcare industry is making efforts to offer and continue offering quality care and increase the credentials that enhance the competitiveness of organizations. Â  Scholars Lundy and Janes in their research argued that the main objective of healthcare administration is to ensure that the delivery of healthcare reaches residents in the efficient ways, to the required standards. Governments ensure that the provision of healthcare ensures continued support in the growth of the economies. This cannot be delivered if the administration and the organization as a whole do not prioritize work-life balance and team building among its employees as well as other stakeholders. Enhancing work-life balance is therefore very crucial to ensuring the success and stability of these organizations, and the companies should invest in promoting and building work-life balance for increased productivity and service delivery. This is a decisive move, applicable in building management and leadership to move in the leadership capacity of the health management and practitioners (Site, 2012). There are three potential models for healthcare organizations. These models a re corporate/business models, the orchestrator and the community model. These models represent the different ways in which hospitals conduct their operations. The corporate model is for hospitals, which are operated as financial entities. These hospitals’ main aim is making profit just like any other business. The orchestrator model is for hospitals, which are operated, similar to airports or malls. In this model, hospitals are looked at as providers of infrastructure for specialized and independent health providers. The final model is the community model where the needs of people come before the finance issues (Site, 2012). Â  

What is a revolution Essay Example | Topics and Well Written Essays - 500 words

What is a revolution - Essay Example From whatever direction you approach it, revolution implies a need to consider the historical events before, during, and after a revolution. What were the precipitating factors that brought about the people’s call for change? How did they proceed to bring about the change? Did the change in fact accomplish the goals in the changes it brought about? There are many good examples of revolution to be considered; the French Revolution, the events of which began in 1787, when the call for change rang out among the French; and ended in 1799, with a new Constitution. Another famous revolution is the American Revolution, which lead to America’s independence from England. A revolution, which has not yet been identified as a revolution, and is one that, while it has been extensively discussed by notable authors and journalists such as Bernard Lewis, in his 2005 commentary entitled Freedom and Justice in the Modern Middle East; is perhaps better known as the War on Terrorism; is the Muslim Islamic revolution that has been ongoing since the time of the Prophet Mohammad (Lewis 1-26). â€Å"†¦Muslims – particularly those in the Middle East – have suffered new experiences that have transformed their vision of themselves and the world and reshaped the language in which they discuss it (1).† The experience of Muslims includes the occupation of Egypt by Napoleon, in 1798 (2). The social norms and French law that Napoleon brought with him and attempted to apply to Egypt’s Muslims, were in stark contrast to the laws of Islam (2). â€Å"The Koran,† Lewis writes, â€Å"for example, makes it clear that there is a duty of obedience: ‘Obey God, obey the Prophet, obey those who hold authority over you (6).’† The Koran also gives instructions concerning under what conditions, in case those conditions are not already made clear by the three previously cited dictates; by saying,

Dissection of Microrna-30Ds Function Roles

Dissection of Microrna-30Ds Function Roles DISSECTION OF MICRORNA-30DS FUNCTION ROLES IN MAMMALIAN PANCREATIC-BETA CELLS By:  Yiping Mao   ABSTRACT MicroRNAs (miRNAs) are a group of small non-coding RNAs (about 21-22 nucleotides long) that fine tune target protein output through messenger RNA degradation or inhibition of its translation. Recent studies showing that miRNAs and their function components respond to cellular stress to maintain steady state physiology of the cells. Since there are thousands of microRNAs existing in all kind of cells, their functional characterization during the normal states or stress conditions is not fully addressed yet. In this thesis, important aspects of pancreatic ÃŽÂ ²-cell function under normal or stress condition such as apoptosis, proliferation, insulin production and release and their regulation by the miRNA were explored. Pancreatic ÃŽÂ ²-cell is a group of insulin producing cells and plays critical role in maintaining glucose hemostasis. By combining mouse and cell line genetic approaches, high-throughput deep sequencing, a list of cell assays and molecular techniques, we have shed light on the novel roles of miR-30d, one highly expressed miRNA when ÃŽÂ ²-cell responding to high glucose stimulus, in regulating ÃŽÂ ²-cell mass on the middle aged mice. We demonstrated that overexpress of miR-30d deteriorated glucose tolerance ability of the mice with or without high fat diet treatment by significantly reducing the ÃŽÂ ²-cell mass with less insulin production. Additionally, we demonstrated that the reduced ÃŽÂ ²-cell mass is because both apoptosis pathway and proliferation p athway have been effected by miR-30d by targeting variety of protein factors expression. BCL2 interacting protein 3 (BNIP3) and cyclin E2 (CCNE2) have been respectively confirmed as miR-30ds targets and the effect of their regulation by miR-30d in pancreatic ÃŽÂ ²-cell proliferation or apoptosis aspects have been addressed as well. Furthermore, we could show that silencing of miR-30d in MIN6 cell (ÃŽÂ ²-cell mimicking cell line) by CRISPR-CAS9 gene editing system promotes the insulin secretion, which is through potentiated expression of MAFA, an insulin transcription factor. These studies uncovered novel functional roles of the miR-30d pathway in mediating ÃŽÂ ²-cell function and fate. Further dissection of these pathways shall uncover several mechanisms by which the ÃŽÂ ²-cells undertake to maximize their efficiency during disease states such as T2D.   CHAPTER 1: INTRODUCTION AND BACKGROUND 1.1 The Brief History of Diabetes and Research Long before terming diabetes, which means to pass through by Greek Apollonius of Memphis in 250 BCE and the re-discovery of honey-like-urine (glycosuria) by Thomas Willis, who included the term mellitus, Diabetes has discovered its first saying around 1500 B.C. in an Egyptian manuscript. It was perceived as a disease related with too great emptying of the urine (polyuria). Later, important discoveries includes Matthew Dobsons first proof of elevated urine and blood glucose levels (hyperglycemia) in people with diabetes (Dobson, 1776). In 1889, Joseph von Mering and Oskar Minkowski were the first to give evidence that pancreas removal in dogs induced diabetes, proposing that pancreas functional related to glucose levels. Afterward, Edward Albert Sharpey-Schafer proposed that diabetes could be brought on by losing a pancreatic chemical, which he named as insulin in 1910 (Polonsky, 2012). Taken together, diabetes is currently well recognized as a gathering of heterogeneous disorders cha racterized by hyperglycemia because of loss of insulin or its effectiveness. Current worldwide trends demonstrate a surprising 382 million individuals have diabetes and this is predicted to ascend to 592 million by the year 2035 (IDF Diabetes Atlas, 2013). The cases of diabetes complications, including diabetic retinopathy, cardiovascular disease and renal failure are constantly rising and the death rate because of those are worsen every year. Claude Bernards identification of liver as the major glucose production organ led to the first concept of homeostasis which has been termed and expanded by Walter Bradford in the mid-nineteenth century, to describe the maintaining of steady-state physiology of the cells (Robin, 1979). This gave the notion that actually the disturbed glucose homeostasis is one of the important events of the diabetes progression. Given the evidences of insulin is involved in maintaining the glucose homeostasis, Frederick Banting and Charles Best integrated a series of scientific approaches, and were able to purify the insulin from the pancreas. Moreover, they successfully treated the patients who suffer from the diabetes, with their purified insulin (Banting Best, 1922; Banting et al., 1922). This landmark finding set the stage for treating the severe diabetes with insulin. However, it has been almost a century now since the first time insulin was discovered and purified, diabetes remains the incurabl e disease, requires life-time attention and treatment because of its complexity. Diabetes has been classified as a couple different types nowadays, the major two types are known as the Type I Diabetes (T1D) or Insulin Dependent Diabetes Mellitus (IDDM) and Type II Diabetes (T2D) or Non-Insulin Dependent Diabetes Mellitus (NIDDM). Surprisingly, these two major types has been notified as early as 100-200 B.C by Indian physicians (Kahn, 1994). However, the first scientific evidence was brought by Wilhelm Falta and Harold Himsworth after they set the standardized glucose/insulin tolerance test in human to distinguish the insulin sensitive from non-sensitive patients (Himsworth, 1936). Insulin dependent T1D is featured in insufficient insulin production due to autoimmune response to the pancreatic beta-cells and it mostly affects young kids. T2D is more described as a metabolic syndrome with emergence of insulin resistance and obesity etc, and it is more common among adults (Moller, 2001). There is a complex network of several insulin responding tissues contributes to the maintenance of glucose homeostasis, and of course any abnormality in this network induces the progression of T2D. Besides the metabolic relevant tissues, the gene-interactions also play critical roles in the development of T2D and obesity such kind of metabolic diseases (Doria et al., 2008). 1.2 The Islet Architecture, ÃŽÂ ²-cell Fate determination The islets of Langerhans are specialized endocrine part of pancreas and are the only part producing the secreting hormones. They basically comprise of various cell sorts named ÃŽÂ ±, ÃŽÂ ², ÃŽÂ ´, PP, and ÃŽÂ µ that secret the islet hormones glucagon, insulin, somatostatin, polypeptide Y, and ghrelin individually, and these hormones are required to maintain the glucose homeostasis at normal or stress state. Furthermore, the islets are known to have dynamic and plastic architecture that is proposed to be adjusted over the time of development (Steiner et al., 2010). During the progression of insulin resistance, pregnancy or T2D, the islets extend in size to make up for expanded insulin requirement (Weir and Bonner-Weir, 2004; Kim et al., 2009). But afterwards, there is a considerable loss of ÃŽÂ ²-cell mass because of environmental or genetic factors, inducing the serious hyperglycemia in cause of insulin deficiency. While islets count to just ~1-2% of the whole pancreas, the insulin producing ÃŽÂ ²-cells represent ~65-80% of the islet mass, constituting to roughly 2% of pancreatic weight. Furthermore, the rest other cell types are considered as non ÃŽÂ ²-cells of the islets (Weir and Bonner-Weir, 2013). During the development, these distinctive cell types are known to emerge from a single progenitor cell that producing Neurogenin3 or Ngn3, a transcription factor that decides endocrine cell destiny (Edlund, 2002). Afterwards, other transcription factors, for example, Pdx1, Pax4, Nkx2.2, Nkx6.1, MafA, and Foxo1 help the ÃŽÂ ²-cell fate determination (Ziv et al., 2013). While it had been learned as that ÃŽÂ ²-cells proliferate by self-duplication from old ÃŽÂ ²-cells instead of differentiation from stem cell (Dor et al., 2004), some other study suggested that multipotent cells inside the pancreas could differentiate into ÃŽÂ ²-cells as well (Xu et al., 2008). The latter study is a lso confirmed by researches showing how expressing the ÃŽÂ ²-cell specific transcription factors in non ÃŽÂ ²-cell could trigger a ÃŽÂ ²-cell lineage in mice (Collombat et al., 2009; Al-Hasani et al., 2013). Recent studies additionally showing that non-ÃŽÂ ² cells, for example, ÃŽÂ ±-and ÃŽÂ ´-cells could experience transdifferentiation into ÃŽÂ ²-like cells when the mice is suffering from significant ÃŽÂ ²-cell loss (Thorel et al., 2010; Chera et al., 2014). On the other hand, a few researches demonstrate that the ÃŽÂ ²-cells can likewise lose their fate or dedifferentiate into non-ÃŽÂ ² or progenitor cells when losing any of the previously mentioned ÃŽÂ ²-cell particular transcription factor (Ziv et al., 2013). Moreover, a current study demonstrated that human ÃŽÂ ²-cells are capable of converting into ÃŽÂ ±-cells with no genetic modification (Spijker et al., 2013). Taken together, all these researches have showed that the dynamic plasticity of islet cells. 1.3 Glucose Stimulate Insulin Secretion One unique and significant feature of ÃŽÂ ²-cells is to detect the blood glucose changes and secrete insulin into extracellular milieu in response to keeping the glucose levels within the range of 4-8mM (Weir and Bonner-Weir, 2013). This is primarily accomplished by the take-up of extracellular glucose by the glucose transporter 2 (Glut2; Bell et al., 1990) at the plasma membrane. Upon uptake, the intracellular glucose sensor, glucokinase (Gck), subjects glucose moieties to quick metabolism system by glycolysis (Matschinsky and Ellerman, 1968). This brings about the producing of three carbon products: pyruvate, which takes part in the tricarboxylic corrosive (TCA) cycle inside the mitochondrion to eventually create adenosine triphosphates (ATP) by means of the electron transport chain system. The ATP therefore leads to the increase of ATP/ADP proportion in the cytoplasm, activating the closure of the ATP sensitive potassium (KATP) channel. Vitally, mutation in the kir6.2 subunit o f this channel were demonstrated to induce neonatal diabetes in both mice and human because of loss of insulin secretion as a consequence of constitutively open KATP channel (Koster et al., 2000; Gloyn et al., 2004). It has long been realized that glucose stimulates the closure of these KATP channels thus leading the slow menbrane depolarization (Ashcroft et al., 1984). This promotes extracellular calcium influx by voltage dependent calcium channels and potentiates the releasing of insulin (Matschinsky et al., 1998). Insulin is secreted in an oscillatory manner because of the blood glucose level and triggers downstream insulin signaling cascade in insulin-responsive tissues for the taking up glucose. It has well been shown that islets can be entrained to small changes in glucose and thus the plasma insulin has high frequency of oscillation. However, this capacity of entrainment of the islets is disturbed in patients with T2D (Mao et al., 1999). It exhibits ÃŽÂ ²-cell malfunction because of loss of insulin secretion is a major issue during the clinical indication of T2D. 1.4 Compensatory Islet Expansion During Insulin Resistance During the state of insulin resistance or over-weight, elevated plasma insulin levels (named hyperinsulinemia) has been found in polygenic mouse models showing insulin resistance and human subjects because of increasing of insulin secretion (Yalow and Berson, 1960; Polonsky et al., 1988; Brà ¼ning et al., 1997). It has been suggested later that both in rodents and people, this improved insulin secretion is apparently because of an expansion in ÃŽÂ ²-cell mass by either ÃŽÂ ²-cell proliferation (Steil et al., 2001) or ÃŽÂ ²-cell hypertrophy (Weir and Bonner-Weir, 2004). On the other hand, ÃŽÂ ²-cell failure because of different genetic or environmental variables, is known to cause declined plasma insulin levels in diabetics (Maclean and Ogilvie, 1955). It has been showed that lessened levels of insulin are frequently associated with a noteworthy loss of ÃŽÂ ²-cell mass because of ÃŽÂ ²-cell apoptosis (Butler et al., 2003; Rhodes, 2005). Other than the diabetes perspective, it has been demonstrated that matured ÃŽÂ ²-cells have long life-span and low proliferative rates at steady state. This is because of a potential limitation of the entry of matured ÃŽÂ ²-cells into cell cycle (Teta et al., 2005; Kushner, 2013). Other than this perception, later study suggested that adult ÃŽÂ ²-cells do have the ability to proliferate (Stolovich-Rain et al., 2012). In light of these findings on ÃŽÂ ²-cell proliferation, a few research groups have revealed various proteins essential for assisting ÃŽÂ ²-cell proliferation on knockout or transgenic mouse. Known cell cycle controllers including Cyclins D1 and D2, Cyclin subordinate kinase 4 (Cdk4), Cdk inhibitors (CKIs) such as Cip/Kip and INK4, transcription factors Retinoblastoma (Rb) and p53, have been proved on genetic mouse models as regulators of ÃŽÂ ²-cell proliferation and survival (Heit et al., 2006). Although transient high glucose has been considered as the result of insufficient insulin secretion or insulin resistance, it has also been revealed to promote the compensate ÃŽÂ ²-cell mass expansion (Bonner-Weir et al., 1989). This hypothesis was further supported recently by another observation, that it is the glucose metabolism, instead the glucose itself that triggers compensatory ÃŽÂ ²-cell proliferation in vivo (Porat et al., 2011). Some other attentions have been centered on the effect of activation of insulin/IRS2 pathway on driving ÃŽÂ ²-cell proliferation. The components of the pathway including IRS2 (Withers et al., 1998) and AKT (Bernal-Mizrachi et al., 2001) were demonstrated to be fundamental for ÃŽÂ ²-cell survival. Moreover, study has shown that the impact of insulin in ÃŽÂ ²-cell proliferation is even stronger when with hyperglycemia (Paris et al., 2003). It is notable that when in the state of severe insulin resistance, the pancreatic islets adjust t hemselves to meet the expanding requirement for producing and secretion more insulin by increasing their ÃŽÂ ²-cell mass (Weir and Bonner-Weir, 2004). Other than the involvement of protein coding genes, a few non-coding RNAs (ncRNAs), mostly microRNAs and long ncRNAs (lncRNAs), have been shown in the diabetogenes list, adding on to the complex genetic architecture of human diabetes. 2.1 The Brief History of MicroRNAs The most recent decade has seen huge attention regarding a new and special class of little ncRNAs such as microRNAs (miRNAs) in regulating the structure and function of ÃŽÂ ²-cells. With the first observation of a miRNA, lin-4 in C. elegans, researchers showed how a gene product encodes two little RNAs, instead of a protein. Besides, they demonstrated that these small RNAs binds to the compensatory sites at the 3 end of untranslated region (UTR) of lin-14, a development related heterochronic gene. This interaction is appeared to negatively regulate the expression of lin-14 by blocking its translation (Lee et al., 1993; Wightman et al., 1993), proposing miRNAs as negative regulators of gene expression. Even since the discovery of microRNAs, a lot of related research results have updated the mechanism of gene regulation to a novel level. miRNAs now are known as a group of small ncRNAs of ~21-22 nucleotides long that can complementary or non-complementary base-pairing the mRNA of protein coding genes, thus to regulate their expression at post-transcriptional level (Bartel, 2004 and 2009). Actually after the identification of lin-4, another miRNA called let-7 was revealed like lin-4 in both biogenesis and function level. Soon after, there starts a prevail in discovering new microRNASs mostly by high throughput sequencing technologies in research area and surprisingly, about 30,000 miRNA over about 200 species have been identified, which includes about 2,500 mature human miRNAs. (Kozomara and Griffiths-Jones, 2014). Many computational methods have also been produced to predict the potential targets of miRNAs based on the stable miRNA-target mRNA binding model (Lewis et al., 2003; Krek et al., 2005). 2.2 MicroRNA Induced Gene Silencing The intercellular gene silencing mechanism, termed as RNA silencing (RNAi) or post-transcriptional gene silencing (PTGS) is currently well known to be led by a group of small RNAs, for example, short interfering RNA (siRNA), piwi interacting RNA (piRNA), or the miRNAs. Basically, their working mechanisms are similar and the difference exist mostly in their biogenesis inside the cells (Ender and Meister, 2010). Mature miRNA producing has been through several steps: transcribed from DNA by RNA polymerase II, primary miRNA (pri-miRNA) has much longer sequence. Once transcribed, the pri-miRNA is further processed to precursor miRNA (pre-miRNA) about 60 nucleotides long by enzyme Dorsa and DGCR8 protein complex (Lee et al., 2003). Once pre-miRNAs are produced inside the nucleus, it will be export out of the nucleus by protein Exportin 5 to the cytoplasm in a Ran-GTP dependent manner (Lund et al., 2004). Another important enzyme, which is also critical for mouse development, Dicer would re cognize the pre-miRNA and process it to about 22 nucleotides long mature miRNA duplex form (Bernstein et al.,2003). Only one of stands of the duplex will be transported to miRNA-induced silencing complex (RISC) by Dicer, and the other strand, termed miRNA* is usually degraded in the end (Schwarz et al., 2003; Ender Meister, 2010). There are several components on the RISC, and one key protein component is AGO family. There are four well characterized members of AGO family in human AGO1, AGO2, AGO3 and AGO4. And AGO2 is expressed more often than other forms (Su et al., 2009; Wang et al., 2012). All the AGO proteins have the ability to slice mRNA because of their PAZ and PIWI cleavage domains under the guidance of miRNA sequence. Each miRNA has an important seed region, typically from 2nd-7th nucleotides, that could fully or partially bind to the mRNA 3 UTR sequence. The base pairing condition between the miRNA and target mRNA determines the target recognition and binding of miRISC, but also the fate of the mRNA- to be cleaved or to be repressed in translation. In animal system, the miRNA does not fully complementary bind to the 3 UTR of the target mRNA, through blocking the translation machinery, miRNA silencing the gene expression, without interference of the target stability. However, recent studies on the miR NA mediated gene silencing in mammalian cells reveals that miRNA may act through two step modes: at first, repressing the translation, then deadenylation and destabilization of target mRNA [27qualifier]. The deadenylation has been suggested to induce mRNA degradation [48] and translation inhibition is probably the requisite of mRNA degradation in mammalian cells [49].

Personal Life and Dear Fellow Students Essay

Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are very important, and yet, necessary component of a happy and healthy life. It helps the undeterred growth of our mind and body at the same time adding smile and tenderness to our daily life. Hence, the value of relaxing and having fun is just as significant as the importance of a dedicated working day at office, or a committed day of learning at school. As the saying goes â€Å"All work and no play makes Jack a dull boy† one must not only work hard but also devote equal time to nature the mental and physical aspect of life. Therefore, in essence, to position ourselves as well-formed, energetic and happy individuals in our society we must develop a balance between our work and entertainment, education and play. After all, what is the use of having a huge amount of wealth or knowledge if one doesn’t have a cheerful personality to enjoy them? Is there anyone out there willing to buy a painting at the cost of his or her eyes†¦? No one, am I right†¦? I am sorry to have chosen a harsh analogy, but in fact, this is what we do when we become mere workaholics. We sacrifice too heavily on something in search of happiness which ultimately turns out to be the very reason for our unhappiness! Respected judges, teachers and my dear fellow students – a very good morning to you all. Be it us, the students, our parents or the global workforce, the contemporary life of all of us has become extremely busy. We have developed kind of an object driven and materialistic attitude towards life and living. To the extent that we care only the marks and money! We don’t, or little realize that ceaseless working or studying reduces our efficiency and make our life dull and depressing. Dear fellow students, physical, recreational and relaxation activities are.